Despite major advances in biomaterials, infections caused by microbial biofilms on device surfaces continue to pose serious health risks. To address this risk of infection, our lab applies thin film mechanics to study biological adhesion. By adapting the laser spallation technique, we quantify the parameters that enhance biofilm adhesion and introduce an innovative Adhesion Index—comparing mammalian cell adhesion to biofilm adhesion. Additionally, we are exploring the structure of biofilm extracellular polymeric substances, also known as the biofilm matrix, which impedes the efficacy of antimicrobial therapies. Our work aims to improve the understanding of microbial mechanics and inform the design of infection-resistant medical surfaces.